Biology Paper 4

Explain the meaning of the term Restriction Endonuclease and state it's role during the production of insulin by genetic engineering (4 marks)

Cystic Fibrosis (CF) is an inherited disease. Explain briefly how two parents who do not have CF may have a child with CF (2 marks)

Describe the ways in which CF affects the lungs (2 marks)

One potential way of treating CF is by using gene therapy. Outline, with reference to CF, what is meant by gene therapy. (2 marks)

Describe one possible advantage and one possible disadvantage of using gene therapy to treat CF (2 marks)

Outline the principles of Gel Electrophoresis (4 marks)

Define the term BIOINFORMATICS (2 marks)

Explain the properties of plasmids which make them suitable for use during genetic engineering (3 marks)