Rotovirus

In the children's infectious disease section of the city hospital was hospitalized group of children with pre-set clinical diagnosis of "gastroenteritis rotavirus." What is the basic principle laboratory diagnostic applies in this case?

• Detection of viral antigen in the test material

• Identification of specific inclusions in the cells.

• Infection of chick embryos

• Isolation of the virus in cell culture with subsequent identification

• Infection of laboratory animals

There are almost all children had gastroenteritis signs in a few hours after cheese curd intake in the kindergarten. At time of bacteriological examination of vomiting masses and wastes of the cheese curd the golden staphylococcus was revealed. How is expedient to continue a research for a specification of diagnosis?

• Make a lysotyping of allocated strains

• Define an ability of strains to produce toxins

• Take a research of nutrition unit equipment

• Define a presence of antibodies at ill children

• Take allergic test

The 6-monthed child was entered to the infectious hospital with diagnosis “acute gastroenteritis”. On what nutrient medium it is necessary to sow faeces of ill child?

• Moncur‟s medium

• Blood agar

• Endo medium

• Ploskirev‟s medium

• Serum agar

The pathogen was isolated from patient‟s organism (patient suffers from acute gastroenteritis). It must be identified by antigen structure. What serologic reaction must be used?

• Reaction of agglutination

• Reaction of complement fixation

• Reaction of neutralization

• Reaction of precipitation

• Reaction of opsonization

The serological test was used for bacterial culture seroindentification in the bacteriological laboratory with the material from a patient with suspected salmonella gastroenteritis. What type of reaction can be supplied with this antigen?

• The reaction of neutralization

• Agglutination

• The reaction of precipitation

• The reaction of indirect

In the feces of a patient with acute gastroenteritis there were revealed motile, slightly curved Gram-negative rods, which grow onto alkaline 1% peptone water in the form of tender bluish pellicle. What microorganisms have such properties?

• Spirilla.

• Spirochetes.

• Clostridia.

• Bacilli.

• Vibrios.

The pure culture of Gramnegative, slightly curved, motile rods that fermented mannose and sucrose to acid and was agglutinated by O1 choleraic anti-sera was isolated from patient with acute gastroenteritis feces. What media was used for isolated pure culture?

• 1% peptide alkaline water, TCBS media

• Blood meat pepton agar, Ploskirev`s medium

• 1% pepton alkaline water, Endo media

• Meat peptide agar, meat pepton broth

• Sugar and meat agar, alkaline meat pepton agar

In the infectious ward three students were brought who lived in the same room, with a diagnosis of "acute gastroenteritis." The disease occurred after ingestion of cooked meat, which remained at room temperature during the day. What is the most probable result of seeding of pathological material (gastric washings and feces) on the culture media?

• The blue colonies on agar, alkaline

• Crimson colonies on Endo medium

• Uncoated colonies on Endo medium

• Yellow colonies on VSA

• Large slimy colonies on MPA