BIO615 quiz 3

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.
• Cambodia
• The rain forest
• The CDC

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

Tags

NGSS.HS-PS1-2

NGSS.HS-PS1-5

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

What applications can gel electrophoresis be used for?

• Parental Testing
• Criminal Investigations
• Sequence Endangered Species DNA
• All of the above

How are buffers used in Electrophoresis?

• Conduct electricity

• Remove salts

• Add extension energy

• Dissolve excess DNA