CRISPR-Cas9 Gene Editing Flashcard

Front

What is CRISPR-Cas9?

Back

A revolutionary gene-editing technology that allows for precise modifications to DNA in living organisms.

Front

What does Cas9 stand for?

Back

CRISPR-associated protein 9, an enzyme that acts as molecular scissors to cut DNA.

Front

What is the primary function of the Cas9 enzyme in bacteria?

Back

To destroy invading viral DNA.

Front

How does CRISPR-Cas9 work?

Back

It uses a guide RNA to direct the Cas9 enzyme to a specific location in the DNA, where it makes a cut.

Front

What is a guide RNA?

Back

A short synthetic RNA that is designed to be complementary to the target DNA sequence.

Front

What is a PAM sequence?

Back

A sequence of two to six nucleotides next to the DNA targeted by Cas9, essential for Cas9 binding.

Front

What is the role of Cas9 in genome editing?

Back

To act as molecular scissors cutting DNA at specific sites.

Front

How can CRISPR-Cas9 be used to correct genetic mutations?

Back

By cutting the mutated gene and allowing repair with a correct sequence.

Front

What are the potential applications of CRISPR-Cas9 technology?

Back

Gene therapy, agriculture, and research in genetics.

Front

What is gene therapy?

Back

A technique that modifies a person's genes to treat or prevent disease.