Flashcards- ABE Lab 2a & 4a

Flashcards- ABE Lab 2a & 4a

Assessment

Flashcard

Science

9th Grade

Easy

Created by

Kelly Jones

Used 1+ times

FREE Resource

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18 questions

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1.

FLASHCARD QUESTION

Front

Overall Purpose of LAB 2a (restriction digest) and LAB 4a (gel electrophoresis)

Back

Lab 2a and 4a are needed to ensure the plasmid is correct for making the red fluorescent protein. Lab 2a uses restriction enzymes to cut the plasmid, and lab 4a is used to verify that these are the correct size fragments for the para-R plasmid.

2.

FLASHCARD QUESTION

Front

Use of restriction enzymes in LAB 2a

Back

A combination of 2 different restriction enzymes is used to digest (hydrolyze) the pBAD-rfp segment out of the plasmid.

3.

FLASHCARD QUESTION

Front

Describe the parts of the pARA-R plasmid

Back

The pARA-R plasmid is 5,302 bp long and includes components like the red fluorescent protein (rfp) gene, the ampicillin resistance gene (ampR), and the arabinose activator (araC).

4.

FLASHCARD QUESTION

Front

True or False: Restriction enzymes are naturally found in Prokaryotes.

Back

True; Restriction enzymes provide protection against invading viruses by hydrolyzing "foreign" DNA.

5.

FLASHCARD QUESTION

Front

Restriction enzymes cut DNA at specific recognition nucleotide sequences called ________

Back

Media Image

6.

FLASHCARD QUESTION

Front

What are "sticky ends"

Back

Media Image

7.

FLASHCARD QUESTION

Front

Which 2 Restriction enzymes did we use in lab 2a to digest the pARA-R plasmid?.

Back

HINDIII and BamHI

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