Overall Purpose of LAB 2a (restriction digest) and LAB 4a (gel electrophoresis)
Flashcards- ABE Lab 2a & 4a

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Science
•
9th Grade
•
Easy
Kelly Jones
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18 questions
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1.
FLASHCARD QUESTION
Front
Back
Lab 2a and 4a are needed to ensure the plasmid is correct for making the red fluorescent protein. Lab 2a uses restriction enzymes to cut the plasmid, and lab 4a is used to verify that these are the correct size fragments for the para-R plasmid.
2.
FLASHCARD QUESTION
Front
Use of restriction enzymes in LAB 2a
Back
A combination of 2 different restriction enzymes is used to digest (hydrolyze) the pBAD-rfp segment out of the plasmid.
3.
FLASHCARD QUESTION
Front
Describe the parts of the pARA-R plasmid
Back
The pARA-R plasmid is 5,302 bp long and includes components like the red fluorescent protein (rfp) gene, the ampicillin resistance gene (ampR), and the arabinose activator (araC).
4.
FLASHCARD QUESTION
Front
True or False: Restriction enzymes are naturally found in Prokaryotes.
Back
True; Restriction enzymes provide protection against invading viruses by hydrolyzing "foreign" DNA.
5.
FLASHCARD QUESTION
Front
Restriction enzymes cut DNA at specific recognition nucleotide sequences called ________
Back
6.
FLASHCARD QUESTION
Front
What are "sticky ends"
Back
7.
FLASHCARD QUESTION
Front
Which 2 Restriction enzymes did we use in lab 2a to digest the pARA-R plasmid?.
Back
HINDIII and BamHI
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