What is a plasmid?
AP Bio Insta-Review Topic 6.8*: BioTechnology

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Biology, Science
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9th - 12th Grade
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Hard
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1.
FLASHCARD QUESTION
Front
Back
A plasmid is a small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently. Plasmids are often used in genetic engineering to transfer genes between organisms.
2.
FLASHCARD QUESTION
Front
What is the purpose of gel electrophoresis?
Back
Gel electrophoresis is a technique used to separate DNA, RNA, or proteins based on their size and charge. It allows scientists to analyze the genetic material.
3.
FLASHCARD QUESTION
Front
What does PCR stand for and what is its function?
Back
PCR stands for Polymerase Chain Reaction. It is a technique used to amplify small segments of DNA, making millions of copies of a specific DNA sequence.
4.
FLASHCARD QUESTION
Front
What is the role of ampicillin in bacterial growth experiments?
Back
Ampicillin is an antibiotic that inhibits the growth of bacteria. In experiments, it is used to select for bacteria that have been genetically modified to be resistant to it.
5.
FLASHCARD QUESTION
Front
How do larger molecules behave in gel electrophoresis?
Back
Larger molecules move more slowly and tend to remain near the wells in gel electrophoresis, while smaller molecules travel farther through the gel.
6.
FLASHCARD QUESTION
Front
What is the significance of the green glow protein in genetic engineering?
Back
The green glow protein, often derived from jellyfish, is used as a marker to indicate successful gene transfer and expression in genetically modified organisms.
7.
FLASHCARD QUESTION
Front
What is the function of restriction enzymes in biotechnology?
Back
Restriction enzymes are proteins that cut DNA at specific sequences, allowing scientists to manipulate DNA for cloning and genetic engineering.
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