F24 H Molecular Genetics FINAL

Front

What is the role of the pGLO plasmid in bacterial transformation?

Back

The pGLO plasmid carries the gene for green fluorescent protein (GFP) and the gene for antibiotic resistance, allowing transformed bacteria to grow in the presence of ampicillin and express fluorescence under UV light.

Front

What is bacterial transformation?

Back

Bacterial transformation is the process by which bacteria take up foreign DNA from their environment, leading to genetic changes.

Front

What is the expected result of bacterial growth on a '+pGLO, LB/amp' plate?

Back

Bacterial growth without biofluorescence, as the bacteria have taken up the plasmid but may not express the GFP gene.

Front

What does LB/amp stand for in microbiology?

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LB stands for Luria-Bertani broth, a nutrient-rich medium for bacterial growth, and amp refers to ampicillin, an antibiotic used to select for transformed bacteria.

Front

What is the significance of using ampicillin in the transformation experiment?

Back

Ampicillin is used to select for bacteria that have successfully taken up the pGLO plasmid, as only those bacteria will survive in its presence.

Front

What is the function of the GFP gene in the pGLO plasmid?

Back

The GFP gene encodes for green fluorescent protein, which allows transformed bacteria to fluoresce under UV light.

Front

What is the purpose of incubating the plates after transformation?

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Incubation allows time for the bacteria to express the genes carried by the plasmid and to grow into visible colonies.

Front

What is the difference between selective media and non-selective media?

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Selective media contains substances that inhibit the growth of certain bacteria while allowing others to grow, whereas non-selective media supports the growth of a wide variety of organisms.

Front

What is the importance of controls in an experiment?

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Controls are essential to validate the results of an experiment by providing a baseline for comparison.

Front

What is the expected result of bacterial growth on a '-pGLO, LB' plate?

Back

Bacterial growth without biofluorescence, as there is no plasmid present.