MI 2.1.3 Review

Front

What is the purpose of the 100 bp ladder in DNA experiments?

Back

To provide a size reference for DNA fragments.

Front

What is the function of restriction enzymes in DNA analysis?

Back

They cut DNA at specific recognition sites.

Front

What equipment is recommended for visualizing DNA after PCR?

Back

UV Transilluminator or Blue Light Visualization System.

Front

How many cycles of PCR are typically run before product levels plateau?

Back

35 cycles.

Front

What is the size of the PCR product for a Homozygous Taster (TT) after Hae III restriction digest?

Back

44 bp and 177 bp.

Front

Define PCR (Polymerase Chain Reaction).

Back

A technique used to amplify specific DNA sequences.

Front

What is the role of DNA polymerase in PCR?

Back

To synthesize new DNA strands by adding nucleotides.

Front

What does SNP stand for in genetic analysis?

Back

Single Nucleotide Polymorphism.

Front

Explain the significance of SNPs in genetics.

Back

SNPs can affect how humans respond to diseases, medications, and environmental factors.

Front

What is the function of a UV Transilluminator in DNA analysis?

Back

To visualize DNA fragments that have been stained with a fluorescent dye.