Gel Electrophoresis

Gel Electrophoresis

Assessment

Flashcard

Biology

9th Grade

Medium

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Wayground Content

Used 1+ times

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15 questions

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1.

FLASHCARD QUESTION

Front

What is gel electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

2.

FLASHCARD QUESTION

Front

What is the purpose of adding enzymes to DNA before gel electrophoresis?

Back

To cut the DNA into fragments for analysis.

3.

FLASHCARD QUESTION

Front

How does gel electrophoresis separate DNA fragments?

Back

By applying an electric current, which causes the negatively charged DNA to move through a gel matrix, separating fragments by size.

4.

FLASHCARD QUESTION

Front

What is the role of the gel in gel electrophoresis?

Back

The gel acts as a medium that allows smaller DNA fragments to move faster than larger ones, facilitating separation.

5.

FLASHCARD QUESTION

Front

What are the two main types of gels used in electrophoresis?

Back

Agarose gel and polyacrylamide gel.

6.

FLASHCARD QUESTION

Front

What is the significance of the size of DNA fragments in gel electrophoresis?

Back

Smaller fragments migrate faster and further through the gel than larger fragments, allowing for size estimation.

7.

FLASHCARD QUESTION

Front

What is a DNA ladder in gel electrophoresis?

Back

A mixture of known DNA fragments used as a reference to determine the size of unknown DNA samples.

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