Gel Electrophoresis
Flashcard
•
Biology
•
9th Grade
•
Medium
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15 questions
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1.
FLASHCARD QUESTION
Front
What is gel electrophoresis?
Back
A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.
2.
FLASHCARD QUESTION
Front
What is the purpose of adding enzymes to DNA before gel electrophoresis?
Back
To cut the DNA into fragments for analysis.
3.
FLASHCARD QUESTION
Front
How does gel electrophoresis separate DNA fragments?
Back
By applying an electric current, which causes the negatively charged DNA to move through a gel matrix, separating fragments by size.
4.
FLASHCARD QUESTION
Front
What is the role of the gel in gel electrophoresis?
Back
The gel acts as a medium that allows smaller DNA fragments to move faster than larger ones, facilitating separation.
5.
FLASHCARD QUESTION
Front
What are the two main types of gels used in electrophoresis?
Back
Agarose gel and polyacrylamide gel.
6.
FLASHCARD QUESTION
Front
What is the significance of the size of DNA fragments in gel electrophoresis?
Back
Smaller fragments migrate faster and further through the gel than larger fragments, allowing for size estimation.
7.
FLASHCARD QUESTION
Front
What is a DNA ladder in gel electrophoresis?
Back
A mixture of known DNA fragments used as a reference to determine the size of unknown DNA samples.
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