Blackett Family DNA Activity Flashcard

Front

What is the function of DNA ladders?

Back

To determine the length of the DNA in bands in other lanes.

Front

What is the main purpose of autorads in DNA analysis?

Back

To compare DNA bands.

Front

What primary technique is used for DNA profiling in the Blackett family?

Back

RFLP analysis.

Front

Which generation is represented in the back row of the Blackett family photo?

Back

Grandparents.

Front

What do autorads represent in the Blackett Family DNA Activity?

Back

X-ray films with dark bands representing RFLP markers.

Front

Define RFLP analysis.

Back

Restriction Fragment Length Polymorphism (RFLP) analysis is a technique used to compare the lengths of DNA fragments produced by restriction enzyme digestion.

Front

What is the role of PCR in DNA analysis?

Back

Polymerase Chain Reaction (PCR) is used to amplify small segments of DNA, making millions of copies for analysis.

Front

Explain the significance of DNA bands in gel electrophoresis.

Back

DNA bands indicate the presence and size of DNA fragments, allowing for comparison between samples.

Front

What does the term 'autoradiography' refer to?

Back

Autoradiography is a technique that uses X-ray film to visualize radioactive materials, such as DNA fragments.

Front

What is the purpose of using X-ray films in DNA analysis?

Back

X-ray films capture the patterns of DNA bands, allowing for visual comparison and analysis.