PLTW Medical Interventions Unit 4

Front

What is the purpose of column chromatography?

Back

To purify substances by separating them based on their different interactions with a stationary phase.

Front

What component of recombinant DNA acts as the electricity?

Back

IGTP (Inositol 1,4,5-trisphosphate) is involved in signaling pathways.

Front

What is a laparoscopy?

Back

A minimally invasive surgical procedure that uses a small camera inserted through a small incision.

Front

How many stages of end stage renal disease are there?

Back

There are 5 stages of end stage renal disease.

Front

What is the function of the T7 promoter in GFP?

Back

It acts as an on and off switch for gene expression.

Front

Define recombinant DNA.

Back

DNA that has been artificially made by combining DNA from different organisms.

Front

What is the role of ampicillin in recombinant DNA technology?

Back

Ampicillin is used as a selective agent to ensure that only bacteria containing the plasmid survive.

Front

Explain the significance of the GFP (Green Fluorescent Protein).

Back

GFP is used as a marker in molecular biology to visualize gene expression.

Front

What is the function of a promoter in gene expression?

Back

A promoter is a region of DNA that initiates transcription of a particular gene.

Front

Describe the process of gene cloning.

Back

Gene cloning involves creating copies of a specific gene by inserting it into a vector and introducing it into a host organism.