Gel electrophoresis

Front

What is gel electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What is the role of enzymes in gel electrophoresis?

Back

Enzymes are used to cut DNA into fragments for analysis.

Front

How does gel electrophoresis separate DNA fragments?

Back

It separates DNA fragments based on size; smaller fragments move faster through the gel than larger ones.

Front

What is the purpose of using a gel in gel electrophoresis?

Back

The gel acts as a medium through which the DNA fragments can migrate, allowing for separation.

Front

What are the two main types of gels used in electrophoresis?

Back

Agarose gel and polyacrylamide gel.

Front

What does the term 'migration' refer to in gel electrophoresis?

Back

Migration refers to the movement of DNA fragments through the gel when an electric current is applied.

Front

What is the significance of the buffer solution in gel electrophoresis?

Back

The buffer solution maintains the pH and provides ions that carry the current during electrophoresis.

Front

What is a DNA ladder in gel electrophoresis?

Back

A DNA ladder is a set of known DNA fragments used as a reference to determine the size of unknown DNA fragments.

Front

What happens to larger DNA fragments during gel electrophoresis?

Back

Larger DNA fragments move slower and do not travel as far as smaller fragments.

Front

What is the visual representation of DNA fragments after gel electrophoresis called?

Back

A gel image or gel banding pattern.