PCR Review Flashcard

Front

Describe the process of PCR amplification.

Back

PCR amplification is a technique used to make multiple copies of a specific DNA segment.

Front

What is the purpose of PCR?

Back

To produce millions of copies of a specific region of DNA

Front

Steps involved in the Polymerase Chain Reaction process.

Back

Denaturation, Annealing, and Extension

Front

What is the significance of the annealing step in the PCR process?

Back

It allows the primers to bind to the DNA template.

Front

What is the denaturation step?

Back

separating DNA helix with using heat

Front

At what temperature does the Denature step of PCR occur?

Back

94-96° C

Front

Factors to consider when designing PCR primers.

Back

Primer length, GC content, melting temperature, specificity, and avoiding self-complementarity

Front

What is the role of DNA polymerase in PCR amplification?

Back

It synthesizes new DNA strands by adding nucleotides to the template DNA.

Front

Identify and explain two common issues encountered during PCR troubleshooting.

Back

Non-specific amplification and primer-dimer formation

Front

How can non-specific amplification be addressed during PCR troubleshooting?

Back

Optimizing annealing temperature, using specific primers, and adjusting MgCl2 concentration