Microbiology Staining Techniques Quiz

What is the primary focus of the video on differential staining techniques?

• The history of staining techniques

• Advanced microbiology research

• How to physically perform the stains

• Key points likely to be tested in class

In the Gram stain procedure, what is the role of iodine?

• To act as a primary stain

• To decolorize the cells

• To set the crystal violet stain

• To counterstain the cells

Why is it important to use fresh bacterial cultures for Gram staining?

• Old bacteria are more vibrant

• Fresh bacteria are more colorful

• Old bacteria have cell-wall damage

• Fresh bacteria are easier to handle

What is the primary stain used in the Ziehl-Neelsen acid-fast staining method?

• Crystal violet

• Carbol fuchsin

• Safranin

• Methylene blue

What color do acid-fast bacterial cells appear after staining?

• Purple

• Pink or red

• Green

• Blue

Why should you avoid heat-fixing the slide before performing a capsule stain?

• Heat enhances the stain's color

• Heat can destroy the capsule

• Heat makes the stain too dark

• Heat causes the cells to shrink

In Anthony's capsule stain, how do capsules appear under a microscope?

• As red halos

• As white or light-blue halos

• As green halos

• As dark blue halos

What is the primary stain used in the Schaeffer-Fulton endospore staining method?

• Crystal violet

• Malachite green

• Safranin

• Carbol fuchsin

After performing an endospore stain, what color do vegetative cells appear?

• Red

• Blue

• Pink

• Green

What is the purpose of steaming the slide in the endospore staining method?

• To decolorize the cells

• To cool the slide

• To allow the stain to penetrate the spore wall

• To dry the slide quickly