Enzymes Required Practical

What does the enzyme amylase break down?

• Proteins into amino acids

• Fats into fatty acids and glycerol

• Starch into simple sugars

• Cellulose into glucose

What color change indicates the presence of starch when using iodine solution?

• Orange to blue-black

• Blue-black to orange

• Clear to cloudy

• Red to yellow

How is the completion of the starch breakdown reaction indicated in this experiment?

• The solution turns blue-black.

• The iodine solution remains orange.

• The solution becomes cloudy.

• The temperature of the solution drops.

What is the purpose of placing the test tubes in a water bath at 30°C for 10 minutes?

• To denature the enzymes

• To speed up the reaction

• To ensure the solutions reach the correct temperature

• To prevent evaporation of the solutions

What is a problem with taking samples every thirty seconds, and how can it be improved?

• It makes the experiment too long; reduce the water bath temperature.

• It only provides an approximate time for reaction completion; take samples every ten seconds.

• It causes the iodine to denature; use a different indicator.

• It makes the color change too gradual; use a digital colorimeter.

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