Chromatography and Stomata Examination

The video tutorial covers three parts of an AP photosynthesis lab. Part A involves setting up and collecting data on leaf chads in a bicarbonate solution to measure ET50. Part B focuses on chromatography to separate pigments from leaves, using a solvent to calculate RF values. Part C examines stomata from different plant species using a microscope, requiring students to count and draw stomata. Each part includes detailed instructions and data analysis techniques.

What materials are needed for the setup of Part A of the AP photosynthesis lab?

• Colored cups, syringes, bicarbonate solution, spinach leaves

• Test tubes, pipettes, distilled water, lettuce leaves

• Beakers, thermometers, salt solution, kale leaves

• Petri dishes, droppers, sugar solution, cabbage leaves

How many leaf chads are required per setup in Part A?

• 20 chads

• 15 chads

• 12 chads

• 10 chads

What is the purpose of calculating ET50 in Part A?

• To determine the time when all discs have risen

• To estimate the time when 50% of the discs have risen

• To find the time when the first disc rises

• To calculate the average time for all discs to rise

What is the first step in setting up the chromatography experiment in Part B?

• Adding chromatography solvent to the vials

• Heating the solvent

• Mixing pigments with water

• Cutting leaf samples

What is the purpose of using a coin in the chromatography experiment?

• To weigh down the paper

• To measure the solvent level

• To stir the solvent

• To create a pigment line on the paper

How is the RF value calculated in the chromatography experiment?

• By dividing the distance traveled by the solvent by the distance traveled by the pigment

• By multiplying the distance traveled by the solvent by the distance traveled by the pigment

• By subtracting the distance traveled by the pigment from the distance traveled by the solvent

• By adding the distances traveled by the solvent and the pigment

What is the first step in examining stomata in Part C?

• Painting the leaf with clear fingernail polish

• Cutting the leaf into small pieces

• Boiling the leaf in water

• Freezing the leaf

What is used to make an impression of the stomata on the leaf?

• Clear scotch tape

• A microscope slide

• A plastic sheet

• A glass cover slip

What magnification is used to examine the stomata under the microscope?

• 10x

• 40x

• 100x

• 400x

What is the final step in the stomata examination process?

• Calculating the average number of stomata per field of view

• Drawing the leaf structure

• Measuring the leaf length

• Counting the number of leaves