DNA Extraction Process and Techniques

The video tutorial guides viewers through a DNA extraction process. It begins with incubating samples at specific temperatures, followed by adding a protein precipitation solution to separate proteins from the DNA. The samples are then centrifuged to isolate the supernatant, which is transferred to new tubes. Isopropanol is added to precipitate the DNA, and the samples are mixed and centrifuged again to complete the extraction.

What is the total incubation time for the samples before the addition of the protein precipitation solution?

• 60 minutes

• 45 minutes

• 30 minutes

• 15 minutes

What is the purpose of adding the protein precipitation solution?

• To change the color of the solution

• To precipitate proteins

• To lyse the cell membranes

• To dissolve the DNA

Why is it important to wear goggles during the addition of the protein precipitation solution?

• It is a strong acid

• It can be an irritant

• It is highly flammable

• It emits toxic fumes

What should be the orientation of the tubes in the centrifuge?

• Hinges facing outward

• Random orientation

• Hinges facing inward

• Lids facing upward

What is the main reason for balancing the centrifuge?

• To increase speed

• To avoid tube breakage

• To ensure even spinning

• To prevent noise

What is the volume of supernatant transferred to the new tube?

• 200 microliters

• 400 microliters

• 600 microliters

• 800 microliters

Why is it important to avoid debris when transferring the supernatant?

• Debris can change the color

• Debris can contaminate the sample

• Debris can increase the volume

• Debris can dissolve the DNA

What is the role of isopropanol in the DNA extraction process?

• To precipitate DNA

• To dissolve proteins

• To neutralize the solution

• To lyse cell membranes

What visual change indicates DNA precipitation after adding isopropanol?

• Solution becomes clear

• Solution turns blue

• A layer forms at the interface

• Bubbles form in the solution

How long should the samples be spun after adding isopropanol?

• 1 minute

• 5 minutes

• 30 seconds

• 2 minutes