What is the primary goal of site-directed mutagenesis?

Site-Directed Mutagenesis Concepts

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Biology
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11th - 12th Grade
•
Hard

Patricia Brown
FREE Resource
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10 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
To create a new protein from scratch
To change a single amino acid in a protein
To duplicate a protein
To remove a protein from a cell
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which two components are essential for performing site-directed mutagenesis?
A protein and a primer
A cell and a plasmid
A DNA molecule and knowledge of the nucleotide sequence
An enzyme and a substrate
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of creating a DNA primer with a point mutation?
To replicate the entire DNA strand
To delete a section of the DNA
To change the codon sequence for a specific amino acid
To increase the size of the DNA molecule
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is a mismatched base in a DNA primer used in site-directed mutagenesis?
It changes the amino acid sequence of the protein
It prevents the DNA from replicating
It stops the annealing process
It causes the DNA to degrade
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the role of DNA polymerase in site-directed mutagenesis?
To remove the mismatched base
To change the temperature of the reaction
To synthesize a new DNA strand using the primer
To break down the DNA strands
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In the process of site-directed mutagenesis, what happens after the DNA strands are separated?
The DNA is left to cool
The DNA is heated again
The DNA primer is added to the solution
The DNA is discarded
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the significance of using the correct temperature conditions during annealing?
To prevent the DNA from melting
To ensure the DNA strands do not separate
To allow the mismatched base to pair correctly
To facilitate the hybridization of the primer with the DNA
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