Fluorescence Principles and Applications

Fluorescence Principles and Applications

Assessment

Interactive Video

Biology

11th - 12th Grade

Hard

Created by

Patricia Brown

FREE Resource

The tutorial introduces the use of dyes and stains in biological samples, focusing on those with fluorescent properties. It explains the fluorescence process, including excitation and emission, and the cyclical nature of fluorescence. The importance of the light spectrum in fluorescence is discussed, along with the concepts of excitation and emission spectra. The tutorial also covers the potential for photobleaching and the practical applications of fluorescence in detecting microscopic samples.

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10 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary reason fluorescent dyes are favored in biological samples?

They are cheaper than non-fluorescent dyes.

They can be detected with high sensitivity and selectivity.

They are easier to apply to samples.

They do not require any special equipment.

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What happens to a fluorophore when it absorbs light energy?

It loses its ability to fluoresce.

It emits light immediately.

It reaches a higher energy state called an excited state.

It becomes permanently altered.

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the term for the time a fluorophore spends in an excited state?

Fluorescence duration

Excitation period

Excited lifetime

Emission phase

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is photobleaching?

The transition of a fluorophore to its ground state.

The process of a fluorophore emitting light.

The degradation of a fluorophore due to high-intensity illumination.

The absorption of light by a fluorophore.

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How does the wavelength of emitted light compare to the absorbed light in fluorescence?

It is longer.

It varies randomly.

It is shorter.

It is the same.

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the visible spectrum range in nanometers?

300 to 600 nm

500 to 800 nm

400 to 700 nm

200 to 500 nm

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the excitation maximum of a fluorescent dye?

The wavelength at which the dye stops fluorescing.

The wavelength at which the dye is most effectively excited.

The wavelength at which the dye emits the most light.

The wavelength at which the dye absorbs the least energy.

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