What is the primary reason fluorescent dyes are favored in biological samples?

Fluorescence Principles and Applications

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Biology
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11th - 12th Grade
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Hard

Patricia Brown
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10 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
They are cheaper than non-fluorescent dyes.
They can be detected with high sensitivity and selectivity.
They are easier to apply to samples.
They do not require any special equipment.
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What happens to a fluorophore when it absorbs light energy?
It loses its ability to fluoresce.
It emits light immediately.
It reaches a higher energy state called an excited state.
It becomes permanently altered.
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the term for the time a fluorophore spends in an excited state?
Fluorescence duration
Excitation period
Excited lifetime
Emission phase
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is photobleaching?
The transition of a fluorophore to its ground state.
The process of a fluorophore emitting light.
The degradation of a fluorophore due to high-intensity illumination.
The absorption of light by a fluorophore.
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How does the wavelength of emitted light compare to the absorbed light in fluorescence?
It is longer.
It varies randomly.
It is shorter.
It is the same.
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the visible spectrum range in nanometers?
300 to 600 nm
500 to 800 nm
400 to 700 nm
200 to 500 nm
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the excitation maximum of a fluorescent dye?
The wavelength at which the dye stops fluorescing.
The wavelength at which the dye is most effectively excited.
The wavelength at which the dye emits the most light.
The wavelength at which the dye absorbs the least energy.
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