Biological Spill Cleanup Procedures

This video tutorial from the Department of Biological Safety Committee explains the process of cleaning up a biological spill in a laboratory. It outlines six key steps: alerting and informing others, donning personal protective equipment (PPE), preparing disinfectant, executing the cleanup procedure, doffing PPE, and reporting the incident. The video emphasizes the importance of waiting 30 minutes for aerosols to settle before starting the cleanup and provides detailed instructions for each step, including the proper sequence for putting on and removing PPE.

What is the first step in the biological spill cleanup process?

• Cleaning the spill

• Alerting and informing people

• Doffing PPE

• Donning PPE

How long should you wait for aerosols to settle after a spill?

• 10 minutes

• 20 minutes

• 40 minutes

• 30 minutes

Which item is worn first when donning PPE?

• Outer gloves

• N95 respirator

• Safety goggles

• Inner gloves

What should be done to ensure the N95 respirator fits properly?

• Perform a seal check

• Check the expiration date

• Wear it under the lab coat

• Adjust the straps

What is the purpose of using absorbent paper during the spill cleanup?

• To cover the spill

• To mark the spill area

• To dry the area

• To absorb the disinfectant

How should disinfectant be applied to the spill?

• Pour directly onto the spill

• Pour from the center to the outside

• Spray from a distance

• Pour near the spill to avoid splashes

What is the correct sequence for doffing PPE?

• Outer gloves, shoe covers, lab coat, respirator, inner gloves

• Shoe covers, outer gloves, lab coat, eye protection, respirator

• Lab coat, outer gloves, shoe covers, respirator, inner gloves

• Respirator, lab coat, shoe covers, outer gloves, inner gloves

How should biohazard bags be sealed?

• With a zip tie

• With masking tape

• With a staple

• With a rubber band

Who collects the biohazard bags after they are sealed?

• The laboratory staff

• A licensed contractor

• The safety coordinator

• The supervisor

Within how many hours should the incident be reported?

• 12 hours

• 24 hours

• 72 hours

• 48 hours