
Proteolytic Enzymes in Protein Sequencing

Interactive Video
•
Biology, Science, Chemistry
•
11th - 12th Grade
•
Hard

Patricia Brown
FREE Resource
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10 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the first step in protein sequencing?
Using Edman degradation
Breaking down the protein into smaller fragments
Using mass spectrometry
Analyzing the protein with X-ray crystallography
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Why are multiple proteolytic enzymes used in protein sequencing?
To reduce the cost of sequencing
To produce different sets of fragments for overlapping analysis
To increase the speed of the process
To enhance the stability of the protein
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In the example provided, what is the role of the enzyme Tron?
It cleaves at the carboxy end of aromatic amino acids
It cleaves at the amino end of all amino acids
It cleaves at the carboxy end of Lysine and Arginine
It cleaves at the carboxy end of bulky amino acids
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which amino acids does the enzyme Kyrion target?
Bulky and aromatic amino acids
All amino acids
Lysine and Arginine
Only aromatic amino acids
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How many fragments are obtained from the first experiment using Tron?
Four
Three
Two
Five
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the significance of overlapping regions in protein sequencing?
They allow for the correct ordering of fragments
They are used to identify the protein's function
They help in determining the protein's mass
They increase the protein's solubility
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which fragment contains the amino acids Glycine and Tryptophan in the second experiment?
Fragment 1
Fragment 2
Fragment 3
None of the fragments
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