Sanger Sequencing Techniques and Concepts

Sanger Sequencing Techniques and Concepts

Assessment

Interactive Video

Biology, Science, Chemistry

9th - 10th Grade

Hard

Created by

Patricia Brown

FREE Resource

The video tutorial explains the Sanger sequencing method, developed by Frederick Sanger, used to sequence genomes of various organisms, including humans. It details the preparation of target DNA using plasmids, the setup of the sequencing reaction with necessary components like nucleotides and enzymes, and the Sanger reaction process involving temperature changes. The tutorial also covers sorting DNA fragments by size and detecting fluorescently labeled bases, highlighting the method's impact on genetics and health.

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10 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Who developed the Sanger sequencing method?

Rosalind Franklin

Frederick Sanger

Francis Crick

James Watson

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the role of plasmids in the preparation of target DNA?

They act as a template for RNA synthesis.

They serve as vectors to carry target DNA.

They are used to break down bacterial cells.

They provide energy for DNA replication.

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following is NOT added to the sequencing mixture?

DNA nucleotides

Fluorescently labeled nucleotides

RNA polymerase

Primers

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

At what temperature are the DNA strands separated during the sequencing reaction?

50°C

96°C

60°C

37°C

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What causes the termination of DNA strand synthesis in the Sanger method?

Incorporation of a fluorescently labeled nucleotide

Incorporation of a regular nucleotide

Excessive heat

Lack of primers

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How are DNA fragments sorted during the sequencing process?

By their color

By their shape

By their charge

By their size

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is used to detect the fluorescently labeled bases at the end of each fragment?

A laser

A spectrometer

A camera

A microscope

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