Dissection Techniques for Mouse Brain

This video tutorial demonstrates how to dissect the hippocampus from an adult mouse brain. It covers the necessary tools, preparation steps, and detailed dissection process, including separating the brain into halves, exposing the hippocampus, and ensuring compliance with ethical protocols. The tutorial emphasizes careful handling to avoid damage and prepares the tissue for further biochemical experiments or RNA extraction.

What are the essential tools needed for dissecting the hippocampus from a mouse brain?

• A razor blade and a perforated spoon

• A syringe and a pipette

• A scalpel and a microscope

• A drill and a hammer

Why is it important to use a cold surface during the dissection?

• To keep the tools sharp

• To prevent the brain from sliding

• To enhance visibility

• To sterilize the brain

What is the first step in preparing the brain for dissection?

• Separating the brain halves

• Removing the thalamus

• Rinsing the brain in cold PBS

• Cutting the olfactory bulb

How should the brain be positioned to start dissecting the left half?

• With the medial side facing up

• With the ventral side facing up

• With the lateral side facing up

• With the dorsal side facing up

What is the shape of the hippocampus once it is visible?

• Circular

• Triangular

• Banana-shaped

• Square

What should be done to the tissue covering the medial surface of the hippocampus?

• It should be dyed

• It should be frozen

• It should be expanded

• It should be removed

What is the purpose of using a spatula during the dissection?

• To hold down the tissue

• To measure the brain

• To heat the brain

• To cut the brain

What should be inspected for contamination after removing the hippocampus?

• The thalamus

• The midbrain

• The olfactory bulb

• The cerebral cortex

What is the final step before the tissue is ready for experiments?

• Dyeing the tissue

• Inspecting for cortex pieces

• Freezing the tissue

• Weighing the tissue

Which organization approved the animal procedures?

• The European Union

• The World Health Organization

• The Cantonal Veterinary Office of Zurich

• The National Institutes of Health