Mini Prep Process for Plasmid DNA

This video tutorial guides viewers through the process of performing a mini prep to extract and purify plasmid DNA from bacteria. It covers growing bacteria in liquid culture, using centrifugation to collect bacterial pellets, and employing alkaline lysis and column chromatography for DNA purification. The tutorial provides detailed steps for each stage, including resuspension, lysis, and elution, ensuring the plasmid DNA is ready for use or storage.

What is the primary purpose of performing a mini prep?

• To identify bacterial species

• To extract and purify plasmid DNA

• To grow bacteria in solid media

• To measure bacterial growth rate

Which method is used to extract plasmids from bacteria in a mini prep?

• Heat shock method

• Alkaline lysis method

• Conjugation

• Electroporation

What is the first step in preparing a bacterial culture for mini prep?

• Centrifuging the culture

• Adding lysis solution

• Sterilizing the inoculation loop

• Incubating the culture overnight

At what temperature should the bacterial culture be incubated overnight?

• 42 degrees Celsius

• 37 degrees Celsius

• 30 degrees Celsius

• 25 degrees Celsius

What is the purpose of centrifuging the bacterial culture?

• To mix the culture thoroughly

• To pellet the bacteria

• To sterilize the culture

• To increase bacterial growth

How is the bacterial pellet resuspended after centrifugation?

• By adding lysis solution

• By vortexing or flicking the tube

• By heating the tube

• By adding more culture medium

What is added to the tube after the lysis solution to neutralize the reaction?

• Elution solution

• Wash solution

• Neutralization solution

• Resuspension solution

What is the purpose of column chromatography in the mini prep process?

• To neutralize the solution

• To lyse bacterial cells

• To purify plasmid DNA

• To grow bacteria

What is the final step in the mini prep process before storing the purified DNA?

• Adding wash solution

• Spinning the columns

• Discarding the columns

• Adding elution solution

At what temperature can the purified DNA be stored?

• 37 degrees Celsius

• 25 degrees Celsius

• 0 degrees Celsius

• 4 degrees Celsius