What is the primary purpose of performing a mini prep?

Mini Prep Process for Plasmid DNA

Interactive Video
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Biology, Science, Chemistry
•
9th - 10th Grade
•
Hard

Patricia Brown
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10 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
To identify bacterial species
To extract and purify plasmid DNA
To grow bacteria in solid media
To measure bacterial growth rate
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which method is used to extract plasmids from bacteria in a mini prep?
Heat shock method
Alkaline lysis method
Conjugation
Electroporation
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the first step in preparing a bacterial culture for mini prep?
Centrifuging the culture
Adding lysis solution
Sterilizing the inoculation loop
Incubating the culture overnight
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
At what temperature should the bacterial culture be incubated overnight?
42 degrees Celsius
37 degrees Celsius
30 degrees Celsius
25 degrees Celsius
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of centrifuging the bacterial culture?
To mix the culture thoroughly
To pellet the bacteria
To sterilize the culture
To increase bacterial growth
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is the bacterial pellet resuspended after centrifugation?
By adding lysis solution
By vortexing or flicking the tube
By heating the tube
By adding more culture medium
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is added to the tube after the lysis solution to neutralize the reaction?
Elution solution
Wash solution
Neutralization solution
Resuspension solution
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