Tissue Processing Techniques in Pathology Laboratories

The video tutorial explains the process of preparing tissue samples for microscopic examination in a pathology lab. It covers the steps from specimen accessioning, where patient details are recorded, to tissue fixation using formalin. The tutorial describes the use of a tissue processor to replace water in the tissue with paraffin wax, followed by embedding the tissue in a mould. It also explains the use of a microtome to slice the tissue into thin sections, which are then placed on glass slides, stained, and examined under a microscope.

What is the primary goal of tissue processing in a pathology laboratory?

• To enlarge tissue samples for better viewing

• To make tissue samples as thin as paper for microscopic examination

• To destroy unwanted tissue samples

• To color tissue samples for aesthetic purposes

What is the first step in the tissue processing procedure?

• Embedding the tissue in paraffin wax

• Specimen accessioning

• Viewing the tissue under a microscope

• Staining the tissue

Why is it important to assign a unique lab number to each specimen?

• To identify each specimen uniquely for the patient

• To make the specimen look more professional

• To ensure the specimen is not lost

• To prepare the specimen for staining

What is the purpose of fixation in tissue processing?

• To preserve the tissue in a lifelike state

• To make the tissue colorful

• To enlarge the tissue

• To remove the tissue from the body

Which chemical is commonly used as a fixative in tissue processing?

• Acetone

• Chloroform

• Formalin

• Ethanol

What is the role of the embedding center in tissue processing?

• To stain the tissue

• To cut the tissue into thin sections

• To embed the tissue in paraffin wax

• To view the tissue under a microscope

How does a microtome assist in tissue processing?

• By enlarging the tissue

• By embedding the tissue in wax

• By staining the tissue

• By cutting very thin sections of the tissue

What is the purpose of using a water bath in tissue sectioning?

• To stain the tissue

• To heat the tissue

• To smooth out wrinkles in the tissue sections

• To cool the tissue

What is the final step in preparing tissue samples for microscopic examination?

• Staining the tissue and removing the wax

• Accessioning the specimen

• Embedding the tissue in wax

• Cutting the tissue with a microtome

Why is staining important in tissue processing?

• To remove unwanted tissue

• To make the tissue look colorful

• To visualize tissue structures under a microscope

• To preserve the tissue