Settlers fight with Israeli soldiers on illegal settlement

The transcript contains a brief statement, 'No it isn't,' which suggests a negation or disagreement with a previously mentioned idea or statement.

What does the phrase 'No it isn't' imply in a conversation?

In what contexts might someone say 'No it isn't'?

How can the tone of voice affect the meaning of 'No it isn't'?

What are some possible responses to 'No it isn't'?

Can 'No it isn't' be used in a humorous context? Provide examples.