Isocitrate Dehydrogenase

The video explains the mechanism of isocitrate dehydrogenase, focusing on its role in oxidative decarboxylation. It details the conversion of isocitrate to alpha-ketoglutarate, highlighting the involvement of cofactors like manganese and NAD+/NADP+. The process involves oxidation, decarboxylation, and stabilization of intermediates, with a critique of a reaction diagram to clarify the sequence of events.

What are the potential cofactors that can assist isocitrate dehydrogenase aside from manganese?

What is the role of the base in the active site of isocitrate dehydrogenase?

Discuss the transformation of isocitrate to alpha-ketoglutarate and the changes in carbon count.

How does the enzyme regenerate its resting state after the reaction?