PRPP Synthetase Function

The video tutorial explains the synthesis and role of PRPP (phosphoryl pyrophosphate) in nucleotide formation. It details how PRPP is derived from ribose 5 phosphate via the pentose phosphate pathway and its importance in purine and pyrimidine synthesis. The tutorial also covers the configuration of PRPP, emphasizing the SN2 reaction mechanism for nitrogenous base addition, and discusses the regulation of PRPP synthetase.

What is the significance of the nitrogenous base's position in relation to the pyrophosphate group in nucleotides?

How does the addition of the nitrogenous base occur during nucleotide formation?