Affinity Constants

The video tutorial explains the concept of hemoglobin binding with oxygen and introduces the equilibrium reaction between hemoglobin and oxygen. It defines Theta as a measure of protein saturation and discusses equilibrium constants, affinity, and dissociation constants. The tutorial provides methods to calculate protein-ligand concentration using measurable variables and explores the relationship between affinity and dissociation constants. Practical applications of these concepts are also discussed.

What does a high affinity constant (KA) indicate about protein-ligand binding?

What factors can affect the percentage of proteins that are bound by ligands?

Explain the significance of measuring KA and KD in biochemical experiments.

How can you determine the protein-ligand concentration using Theta?