Protease Mechanisms 2

The video tutorial explains the mechanism of caspases, enzymes that induce apoptosis by targeting specific peptide bonds. It details the formation of tetrahedral intermediates and the steps involved in the caspase reaction, including the role of histidine and proton exchanges. The tutorial also compares cysteine and serine proteases, highlighting their similarities and differences in mechanisms.

What role do caspases play in cellular processes?

Describe the significance of the C terminal peptide bond in the action of caspases.

Explain the process of deprotonation of the cysteine residue in the caspase mechanism.

What is a tetrahedral intermediate and how is it formed in the caspase mechanism?

Discuss the role of histidine in the caspase mechanism.

How does the mechanism differ between cysteine proteases and serine proteases?

What happens to the leaving group during the caspase reaction?