Spread Plates | Colony Counts (CFUs/mL) and Difference from Streak Plates

Spread Plates | Colony Counts (CFUs/mL) and Difference from Streak Plates

Assessment

Interactive Video

Science

University

Hard

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The video tutorial explains two techniques for obtaining small isolated colonies: streaking for isolation and spread plates. It covers the mechanical dilution process in streaking and the serial dilution process in spread plates. The tutorial also demonstrates how to calculate colony-forming units (CFUs) and highlights the differences in appearance between streak and spread plates.

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4 questions

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1.

OPEN ENDED QUESTION

3 mins • 1 pt

Discuss the importance of avoiding contamination when isolating bacterial colonies.

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2.

OPEN ENDED QUESTION

3 mins • 1 pt

Why might you choose to use a plate with a dilution of 1 in 10,000 over one with a dilution of 1 in 100,000?

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3.

OPEN ENDED QUESTION

3 mins • 1 pt

What is the significance of the dilution factor in microbiological experiments?

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4.

OPEN ENDED QUESTION

3 mins • 1 pt

What are the visual differences between a spread plate and a streak plate?

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