Spliceosome Assembly and Splicing of mRNA

The video explains the process of splicing in mRNA, focusing on the role of spliceosomes, which are protein-dependent complexes that facilitate the removal of introns and the joining of exons. It contrasts this with self-splicing introns, which do not require proteins. The video details the assembly of spliceosomes, involving small nuclear ribonucleoproteins (snRNPs) like U1, U2, U4, U5, and U6, and the ATP-dependent steps required for their function. The mechanism of splicing, including lariat formation and exon ligation, is described, highlighting the similarities with group 2 self-splicing introns. The video concludes with a brief mention of the next topic, polyadenylation of mRNA.

What are the main differences between self-splicing introns and those that require spliceosomes?

Describe the role of small nuclear ribonucleoproteins (snRNPs) in the splicing process.

Explain the significance of ATP hydrolysis in the assembly of the spliceosome.

What is the function of the spliceosome once it is fully assembled?

How do U1 and U2 snRNPs interact with the intron and exon sequences during splicing?

What is lariat formation and how does it occur during the splicing process?

Discuss the importance of the two prime hydroxyl group in the splicing mechanism.