Detecting Proteins at 280 nm by UV-Vis Why How

The video tutorial explains the use of UV-Vis spectroscopy for protein quantification, focusing on aromatic amino acids like tryptophan and tyrosine. It details the A280 assay method, which measures absorbance at 280 nm to determine protein concentration using the Beer-Lambert law. The tutorial covers plotting absorbance against concentration to find the extinction coefficient and discusses the method's limitations, such as the need for pure protein samples.

Why is it important to ensure that the protein sample is pure when using UV-Vis spectroscopy?

What is the relationship between absorbance and concentration according to Beer's law?

How can you determine the extinction coefficient from a Beer's law plot?

What alternative method is mentioned for protein quantification, and at what wavelength does it operate?