005 The Isoelectric Point of Proteins

This video tutorial explains the concept of the isoelectric point of proteins, which is the pH at which a protein has no net charge. Most proteins in cells have an isoelectric point between 4.5 and 4.7. The video discusses how the pH inside cells, approximately 7.2, affects protein charge, making them negatively charged. The tutorial emphasizes the attraction between opposite charges and concludes with a reminder of the negative charge inside cells due to the isoelectric point and cell pH.

Why is it important to remember the relationship between opposite charges?

What is the isoelectric point of a protein?

What is the average isoelectric point range for most proteins in the cell?

What happens to the charge of proteins when the pH is above the isoelectric point?

How does the pH level affect the charge of proteins in the cell?