Biotechniques | Basics of Making His-Tags & Nickel Affinity Chromatography

The video tutorial explains nickel affinity chromatography, a technique used to separate proteins, particularly those engineered with a his tag. It covers the process of adding his tags to proteins using plasmids and E. Coli, and details the steps of nickel affinity chromatography, including binding, washing, and elution using imidazole. The tutorial highlights the importance of this method in molecular biology and biochemistry for protein purification.

What are the advantages of using a HIS tag for protein expression in E. Coli?

How does imidazole function in the elution process of proteins during nickel affinity chromatography?

Discuss the importance of purification techniques like nickel affinity chromatography in molecular biology.