Protease Mechanisms 3

The video tutorial explores the mechanisms of proteases, focusing on a hypothetical enzyme targeting isoleucine residues. It explains the differences between C terminal and N terminal proteases, and delves into the detailed mechanism of serine proteases, including the formation of tetrahedral and acyl intermediates. The tutorial also compares serine and cysteine proteases, highlighting the role of nucleophiles and the importance of understanding these mechanisms for educational purposes.

What happens to the serine residue during the protease reaction?

What is an ACO enzyme intermediate and why is it important?

How does the mechanism of cysteine proteases compare to that of serine proteases?