AP Biology Lab 6: Molecular Biology

The AP Biology Lab 6 walkthrough covers molecular biology, focusing on bacterial transformation and gel electrophoresis. The lab involves transforming E. coli bacteria using a plasmid called pGLO, which allows the bacteria to glow under a black light. The process includes using calcium chloride and heat shock to facilitate DNA uptake. Gel electrophoresis is used to separate DNA fragments by length. The lab demonstrates how plasmids can confer antibiotic resistance and how different conditions affect bacterial growth. The video provides a detailed explanation of the experimental setup, protocol, and expected outcomes.

What is gel electrophoresis and how is it used in this lab?

How does the presence of arabinose sugar affect the expression of the fluorescent protein?

What outcomes can be expected if bacteria do not pick up the plasmid during the experiment?