Amylase and pH Experiment - Observing Results

The video tutorial demonstrates an experiment to observe the effect of pH on amylase activity. Catherine conducts the experiment using buffer solutions of different pH values, starch, and amylase. The setup includes a water bath to maintain temperature, and spotting tiles to test for starch presence. Results are recorded by observing color changes in iodine, indicating starch digestion. The experiment is repeated for pH values 5, 6, 7, and 8, showing varying digestion times.

What is the purpose of using separate pipettes for each solution in the experiment?

• To maintain the temperature

• To speed up the process

• To avoid cross-contamination

• To measure the exact volume

Why is the Bunsen burner initially set to a yellow flame?

• To heat the water quickly

• To measure the pH level

• To ensure safety while setting up

• To maintain a constant temperature

What is the role of iodine solution in the experiment?

• To maintain pH level

• To act as a buffer

• To test for the presence of starch

• To measure temperature

At what point does Catherine start the stopwatch during the experiment?

• When the buffer solution is added

• When the starch solution is added

• When the amylase solution is added

• When the test tube is placed in the water bath

How long did it take for the starch to be completely digested at pH-5?

• 2.5 minutes

• 2 minutes

• 3 minutes

• 1 minute

What was the recorded time for starch digestion at pH-6?

• 30 seconds

• 1 minute

• 1.5 minutes

• 2 minutes

At which pH level did the starch take the longest time to digest?

• pH-6

• pH-5

• pH-8

• pH-7

What is the significance of maintaining the temperature at 25 degrees centigrade during the experiment?

• To ensure the solutions mix properly

• To prevent the solutions from evaporating

• To provide optimal conditions for enzyme activity

• To maintain the color of the iodine

Why is it important to clean the glass rod between samplings?

• To ensure accurate timing

• To measure the exact volume

• To prevent contamination

• To maintain the temperature

What conclusion can be drawn from the experiment regarding the effect of pH on amylase activity?

• Amylase works best at pH-5

• Amylase activity varies with pH

• Amylase works best at pH-6

• Amylase activity is unaffected by pH