Macroautophagy | Transcriptional Regulation: Lesson 1

The video tutorial discusses the regulation of macroautophagy, focusing on transcriptional activators. It covers various transcription factors like TFEB, E2F1, ATF4, FOXO3, NRF2, HIF1, P53, and PPAR Alpha, explaining their roles in regulating macroautophagy through transcriptional regulation. The tutorial also highlights the conditions under which these factors are activated and their specific targets within the macroautophagy pathway.

Which transcription factor is known as the master regulator of lysosomal biogenesis and autophagy function?

• FOXO3

• NRF2

• TFEB

• E2F1

What happens to TFEB during fasting and starvation?

• It becomes dephosphorylated

• It remains phosphorylated

• It binds to 14-3-3 protein

• It exits the nucleus

Which protein is upregulated by both E2F1 and ATF4?

• BNIP3

• P62

• ULK1

• LC3

How is FOXO3 regulated in the macroautophagy pathway?

• Activated by PI3K

• Inhibited by P53

• Inhibited by AKT

• Activated by NF Kappa B

What role does NRF2 play in macroautophagy?

• Inhibits BNIP3

• Activates TFEB

• Upregulates P62

• Inhibits LC3

Which condition activates HIF1?

• Nutrient abundance

• DNA damage

• Mild hypoxia

• Severe hypoxia

What is the primary function of P53 in the context of macroautophagy?

• Inhibits TFEB

• Activates BNIP3

• Upregulates ATG proteins

• Inhibits FOXO3

During which state is PPAR alpha activated?

• Fasting state

• Fed state

• Hypoxic state

• Starvation state

Which transcription factor is inhibited by FXR during the fed state?

• NRF2

• HIF1

• PPAR alpha

• FOXO3

What is the effect of PPAR alpha on TFEB levels?

• Decreases TFEB levels

• Increases TFEB levels

• No effect on TFEB

• Inhibits TFEB activation