AP Biology Labs - part 2

Mr. Andersen presents part two of the AP Biology Labs, covering bacterial transformation with pGLO plasmid, DNA restriction analysis, energy dynamics in ecosystems, transpiration, animal behavior, and enzyme activity. Each lab is explained with detailed procedures and expected outcomes, providing a comprehensive understanding of these biological processes.

What is the purpose of the arabinose operon in the pGLO plasmid?

• To provide resistance to antibiotics

• To increase the growth rate of bacteria

• To act as an on/off switch for gene expression

• To enable the bacteria to glow under UV light

In the bacterial transformation experiment, what is the expected result on a Petri dish with Luria broth and ampicillin?

• Bacteria glowing under UV light

• Colonies of transformed bacteria

• No bacterial growth

• A bacterial lawn covering the entire dish

What is the role of restriction enzymes in gel electrophoresis?

• To bind DNA to the gel

• To stain DNA for visualization

• To cut DNA into smaller fragments

• To amplify DNA fragments

How can gel electrophoresis be used in forensic science?

• To measure the height of a suspect

• To match DNA samples from a crime scene

• To determine the gender of a suspect

• To identify the age of a suspect

What does a pyramid of energy represent in an ecosystem?

• The total biomass at each trophic level

• The diversity of species at each trophic level

• The number of organisms at each trophic level

• The energy available at each trophic level

How is net primary productivity calculated in the experiment?

• By measuring the carbon dioxide produced

• By adding the initial and final oxygen levels

• By subtracting the initial oxygen level from the final oxygen level

• By measuring the total biomass produced

What is the function of stomata in plant leaves?

• To store water for the plant

• To facilitate gas exchange and water evaporation

• To protect the leaf from herbivores

• To absorb nutrients from the soil

Which factor would likely increase the rate of transpiration in plants?

• High humidity

• Low light intensity

• Strong wind

• Cool temperatures

In the animal behavior experiment, what does kinesis refer to?

• Avoidance of a specific area

• Aggressive behavior towards other animals

• Random movement in response to a stimulus

• Movement towards a specific stimulus

What is the primary function of catalase in enzyme activity experiments?

• To bind substrates together

• To convert glucose into energy

• To break down hydrogen peroxide into water and oxygen

• To synthesize new proteins