Boris Johnson at Francis Crick Institute

The video tutorial covers various scientific concepts and techniques. It begins with an introduction to vacuum and pressure, followed by an analogy of plastic resin to amber. The zebrafish is presented as a model organism for studying development and muscle formation. The tutorial then explains the use of diamond knives in microscopy, emphasizing the care needed due to their cost and fragility. Finally, it discusses the process of slicing tissue into thin sections for observation under a microscope.

What is the advantage of using zebrafish as a model organism in research?

• They are large and easy to handle.

• They have a long lifespan.

• They are transparent, allowing easy observation of development.

• They are inexpensive to maintain.

Why is it necessary to cut tissue into extremely thin slices for microscopy?

• To make the tissue more colorful.

• To reduce the cost of the experiment.

• To fit more samples on the microscope slide.

• To allow the electron beam to pass through the tissue.

What tool is used to cut the tissue into thin slices for microscopic examination?

• A laser cutter

• A scalpel

• A diamond knife

• A ceramic blade

What is a potential consequence of mishandling a diamond knife?

• It could chip, leading to expensive replacements.

• It could rust and become unusable.

• It could become dull and ineffective.

• It could cause injury to the user.

What is the approximate thickness of the tissue slices prepared for microscopy?

• 10 micrometers

• 1 micrometer

• 100 nanometers

• 10 nanometers