Protein Purification Table Calculations: With Numbers
Interactive Video
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Science, Chemistry
•
University
•
Hard
Wayground Content
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7 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the primary purpose of the activity assay described in the video?
To determine the specific activity of enzyme A
To measure the concentration of enzyme Z
To create a standard curve for enzyme A
To calculate the extinction coefficient of enzyme Z
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is the UPM calculated from the activity assay data?
By multiplying the change in absorbance per second by conversion factors
By subtracting the initial absorbance from the final absorbance
By adding the extinction coefficient to the total volume
By dividing the total volume by the extinction coefficient
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the significance of the Bradford assay in the context of this video?
It determines the enzyme's concentration
It calculates the enzyme's extinction coefficient
It provides the enzyme's molecular weight
It measures the enzyme's activity over time
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is the total activity of the enzyme calculated?
By subtracting the concentration from the UPM
By adding the UPM to the concentration
By multiplying the UPM by the total volume
By dividing the UPM by the total volume
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the dilution factor used in the Bradford assay calculation?
1 to 5
1 to 10
1 to 20
1 to 50
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is specific activity determined in this video?
By adding the UPM to the total activity
By dividing the total activity by the concentration
By multiplying the UPM by the concentration
By dividing the UPM by the concentration
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What future calculations are mentioned at the end of the video?
Fold purity and percent yield
Enzyme stability and solubility
Protein structure and function
Molecular weight and enzyme kinetics
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