Phosphodeoxyribomutase: Physiology, Biochemistry, and Mechanism

The video tutorial explains the catabolic pathway of deoxyribose 1 phosphate, focusing on the conversion to deoxyribose 5 phosphate using phospho deoxyribose mutase. It details the enzyme's mechanism, highlighting the role of phosphorylated serine in nucleophilic substitution. The tutorial also introduces deoxyribose 5 phosphate aldolase, which cleaves carbon bonds to produce glyceraldehyde 3 phosphate and acetaldehyde, potentially feeding into the TCA cycle.

What is the initial product formed by purine nucleoside phosphorylase when reacting with a deoxynucleoside?

• Deoxyribose 1 phosphate

• Acetyl CoA

• Glucose 1 phosphate

• Deoxyribose 5 phosphate

Which enzyme is responsible for converting deoxyribose 1 phosphate to deoxyribose 5 phosphate?

• Aldehyde dehydrogenase

• Phospho deoxyribose mutase

• Deoxyribose 5 phosphate aldolase

• Purine nucleoside phosphorylase

What type of reaction is catalyzed by phospho deoxyribose mutase?

• Hydrolysis

• Oxidation

• Isomerization

• Phosphorylation

What are the products of the reaction catalyzed by deoxyribose 5 phosphate aldolase?

• Glyceraldehyde 3 phosphate and acetaldehyde

• Deoxyribose 1 phosphate and water

• Glucose and fructose

• Acetyl CoA and carbon dioxide

What is the role of phosphorylated serine in the enzyme mechanism?

• It stabilizes the enzyme structure

• It acts as a substrate

• It serves as a nucleophile

• It is a leaving group

Which intermediate is neglected in the explanation of the enzyme mechanism?

• Tetrahedral intermediate

• Pentavalent intermediate

• Radical intermediate

• Carbanion intermediate

What is the final product formed after the complete enzymatic reaction involving deoxyribose 1 phosphate?

• Glyceraldehyde 3 phosphate

• Deoxyribose 5 phosphate

• Glucose 6 phosphate

• Acetyl CoA