Micropipette Usage and Techniques

The video tutorial explains the use of micropipettes for measuring small volumes, focusing on the P1000 and P20 models. It covers how to read and set the volume on these pipettes, emphasizing the importance of staying within the designated volume range to avoid damage. The tutorial demonstrates the correct use of pipette tips and the plunger mechanism for aspirating and expelling fluids, highlighting the two-stop plunger system for precise fluid handling.

What is the primary use of micropipettes?

• To measure small volumes, usually less than a milliliter

• To heat liquids

• To measure large volumes of liquid

• To mix chemicals

Which micropipette is used to measure volumes from 100 to 1,000 microliters?

• P100

• P10

• P20

• P1000

How is the volume set on a P20 micropipette?

• By turning a black wheel

• By pressing a button

• By using a digital screen

• By shaking the pipette

What should you never do when adjusting the pipette volume?

• Use a different pipette tip

• Set it to the maximum range

• Turn the wheel too slowly

• Adjust it outside of its designed range

What is the first step in using a micropipette to take up fluid?

• Press the plunger to the second stop

• Place the pipette in the fluid

• Attach a disposable plastic pipette tip

• Set the volume to zero

How do you aspirate fluid into the pipette tip?

• Press the button repeatedly

• Shake the pipette

• Push the button to the first stop and release slowly

• Push the button to the second stop

What should you ensure when drawing fluid into the pipette?

• The tip is fully submerged

• The tip is barely touching the fluid

• The tip is not touching the fluid

• The tip is above the fluid

What is the correct way to expel liquid from the pipette tip?

• Press the button to the second stop

• Press the button halfway

• Press the button to the first stop only

• Shake the pipette

Why is it important to press the plunger to the second stop when expelling liquid?

• To ensure the pipette is clean

• To remove the last bit of fluid from the tip

• To reset the pipette

• To calibrate the pipette

What might happen if you release the button before the tip is out of the fluid?

• The fluid will evaporate

• The pipette will reset

• The fluid may be aspirated back into the tip

• The pipette will break