apps of biotech unit 1

In PCR what role does the template DNA play?

• Serves as reference sequence

• stabliizes the reaction temperature

• initiates the reaction

• Provides the target for amplification

When replicating DNA in the lab, what is used to indicate the starting place for synthesis?

• DNA polymerase

• Template

• dNTPs

• Primers

these are the steps for PCR, which of these is the correct sequence

1.Primer annealing at a lower temp

2.Cooling to allow primers to bind to the template DNA

3. 3. DNA denaturation at 95 degrees C

4. 4.Extension of DNA strands by DNA polymerase

5. 5. Warming to enable DNA synthesis

• 3, 1, 2, 4, 5

• 1, 5, 4, 3, 2

• 5, 3, 2, 1, 4

What is the purpose of nucleotides in a PCR reaction?

• To hold DNA strands apart

• To amplify DNA

• To build the new DNA strands

• To provide the template

What is the function of DNA primers in a PCR reaction?

• The copy RNA

• They provide heat resistance

• They hold DNA strands apart

• They initiate DNA synthesis

Which reagent ensures that DNA polymerase remains active during the high-temperature cycles of PCR?

• Primers

• Buffer Solution

• Nucleotides

• Taq polymerase

What is the function of the phosphate groups in dNTPs during DNA synthesis

• initiating the formation of hydrogen bonds

• determining the specificity of base pairing

• stabilizing the DNA double helix

• providing energy for the reaction

What does the denaturation step in PCR involve

• Separating DNA strands

• Cooling the reaction mixture

• Adding primers

• Extending DNA strands

What is the purpose of a master mix in a polymerase chain reaction?

• to amplify the target DNA sequence

• to serve as a control DNA sample

• to contain all essential components except template DNA and primers

• to provide a template for DNA synthesis

Match this function to the enzyme:

adds nucleotides

• DNA polymerase

• helicase

• RNA primase

• topoisomerase

• ligase