Recombinant DNA

Host Cell

• Organism that is used to receive the recombinant DNA for cloning purpose.

• A type of virus that infects bacteria.

• A cell that produces antibodies.

• An organism that provides nutrients for other organisms.

Define recombinant DNA technology

• Recombinant DNA technology involves the techniques of manipulating DNA by inserting genes from other organisms into another DNA to alter the characteristics of an organism.

• Recombinant DNA technology is a method used to clone entire organisms from a single cell.

• Recombinant DNA technology refers to the process of creating new species through natural selection.

• Recombinant DNA technology is the study of how DNA sequences evolve over time.

What is recombinant DNA?

• A type of DNA found only in bacteria.

• A form of DNA molecule made in vitro by combining genes from different organism/sources.

• A natural DNA sequence that has not been altered.

• A synthetic DNA created solely in a laboratory without any biological components.

What is a plasmid?

• A small, circular piece of DNA that can replicate independently.

• A type of protein found in cells.

• A virus that infects bacteria.

• A component of the cell membrane.

Target DNA / Target Gene

• A segment of RNA that codes for a protein.

• A fragment of DNA containing gene of interest to be cloned that will be inserted into the host cell to produce desired protein.

• A type of protein that regulates gene expression.

• A sequence of nucleotides that does not code for any protein.

Restriction Enzyme

• Enzymes that recognize and cleave/cut DNA into DNA fragments at specific base sequences of DNA called restriction site.

• Proteins that synthesize DNA from RNA templates.

• Enzymes that repair damaged DNA by joining broken strands.

• Molecules that inhibit the replication of DNA during cell division.

Modifying Enzymes

• Enzyme that catalyzes the joining of foreign DNA fragments containing gene of interest and DNA cloning vector to form recombinant DNA molecule.

• Enzyme that breaks down proteins into amino acids.

• Enzyme that synthesizes RNA from a DNA template.

• Enzyme that catalyzes the hydrolysis of lipids.

What is a DNA Cloning Vector?

• A type of protein that helps in DNA replication.

• A DNA molecule that can carry target gene into host cell and replicate there in genetic engineering.

• A method used to sequence DNA.

• A virus that infects bacteria.