Molecular Laboratory Practice Quiz

What is the primary purpose of a Biosafety Cabinet (BSC)?

• To mix reagents

• To amplify DNA

• To provide a safe workspace for handling pathogens

• To store samples and reagents

Which type of Biosafety Cabinet provides maximum protection?

• Class I

• Class II

• Class III

• Class IV

What should be done before using a Biosafety Cabinet?

• Remove waste from the cabinet

• Use proper aseptic techniques

• Wipe surfaces with 70% ethanol or 10% bleach

• Turn off the blower

Which of the following is a key component of PCR machines?

• Vortex mixer

• Refrigerator

• Gel electrophoresis unit

• Heating block

What is the function of a PCR workstation?

• To mix reagents

• To provide a clean area for PCR steps

• To amplify DNA

• To store samples

What is a best practice when using a PCR workstation?

• Use shared pipettes

• Wear gloves and change them regularly

• Avoid cleaning before use

• Crowd the workspace

How does a microcentrifuge function in a molecular lab?

• It controls temperature

• It quantifies nucleic acids

• It separates samples

• It amplifies DNA

Why is regular decontamination important in a molecular lab?

• To save time

• To protect personnel and sample integrity

• To increase equipment efficiency

• To reduce costs

What is the role of a NanoDrop/Spec in a molecular lab?

• To control temperature

• To mix reagents

• To quantify nucleic acids

• To separate DNA/RNA

Which of the following is NOT a type of Biosafety Cabinet?

• Class I

• Class II

• Class V

• Class III