Plant Tissue Culture

What is the primary definition of tissue culture according to the provided text?

• In vivo cultivation of plant cells in natural environments.
• Invitro culture plant cells/tissue under aseptic and controlled conditions
• The process of modifying plant genes in a laboratory.
• Traditional methods of plant propagation in soil.

Which of the following is NOT listed as an advantage of tissue culture technique over conventional cultivation methods?

• Availability of raw material
• Fluctuation in supplies and quality
• Easy purification of compounds
• Dependence on seasonal changes

The concept of "totipotency" suggests that a cell has the potential to develop into what?

• A specialized organ within the plant.
• An entire organism by regeneration.
• A mutated cell line for genetic research.
• Only callus tissue.

Who is credited with the hypothesis in 1902 that a cell is capable of autonomy and has the potential for totipotency?

• White
• Hannig
• Haberblandt
• Simon

What specifically can be patented for plant tissue culture technique?

• Naturally occurring plants or their metabolites as such.
• A novel method of isolation or improvement of yield.
• The chemical structure of natural products.
• The discovery of a new plant species.

Which of the following is considered an "explant" in plant tissue culture?

• The entire mature plant.
• A portion of the plant body taken to establish a culture.
• The soil used for planting.
• The nutrient medium itself.

What essential equipment is primarily used to sterilize culture media in a tissue culture laboratory?

• Hot air oven
• pH meter
• Autoclave
• Shaker

For surface sterilization of explants which of the following common agents is typically used in a solution?

• Pure water
• Sodium hypochlorite
• Sugar solution
• Plant growth hormones

After explant preparation and surface sterilization , what is the next step of in vitro plant culturing?

• Sub-culturing the callus.
• Production of callus from explant.
• Preparing suspension culture.
• Storing the explant in a refrigerator.

In a tissue culture laboratory an aseptic chamber aims to make the working surface free from what?

• Excess humidity
• Dust particles/micro-contaminants
• Too much light
• High temperatures