DNA, RNA, and Transcription Worksheet

Base pair-nucleotide content is calculated by which of the following methods?

• By counting the number of base pairs and multiplying by two to get the nucleotide content

• By dividing the number of nucleotides by two to get the base pair content

• By adding the number of purines and pyrimidines only

• By subtracting the number of guanine from cytosine

What is the Central dogma? What does it refer to?

• Transcription and Translation

• Replication and Mutation

• Cell Division and Differentiation

• Protein Folding and Degradation

A DNA template strand is used to create a complementary pairing from a DNA strand from a promoter region by:

• Using base pairing rules to synthesize a complementary RNA strand during transcription.

• Directly copying the DNA strand without any changes.

• Pairing adenine with guanine and cytosine with thymine.

• Synthesizing a complementary DNA strand using uracil instead of thymine.

What are successive nucleotides (monomers) linked by?

• 3’ – 5’ phosphodiester bonds

• Hydrogen bonds between bases

• Peptide bonds

• Glycosidic bonds

The sequence that an RNA transcript would form from the template DNA strand is:

• complementary to the template DNA strand, replacing T with U

• identical to the template DNA strand, except T is replaced by U

• the same as the coding DNA strand, with T replaced by U

• a random sequence unrelated to the DNA template

What does transcription of RNA from DNA require in eukaryotes?

• A promoter sequence, RNA polymerase, transcription factors

• A ribosome, tRNA, and DNA ligase

• A start codon, release factor, and helicase

• A primer, DNA polymerase, and Okazaki fragments

• A spliceosome, ribosomal subunits, and telomerase

Where do successive ribonucleotides attached to the RNA chain come from during transcription?

• Nucleoside triphosphates (NTPs)

• A promoter sequence, RNA polymerase, transcription factors

• Amino acids

• Deoxyribonucleotides

What is an exon?

• Coding sequence in the mature mRNA that leaves the nucleus

• Non-coding sequence removed during RNA splicing

• A type of enzyme involved in DNA replication

• A region of DNA that codes for tRNA

Is the Promoter Sequence in DNA required for the transcription/initiation process?

• Yes, this is correct.

• No, it is not required.

• Only during DNA replication.

• Only in prokaryotes, not in eukaryotes.

The Poly(A) tail is:

• a stretch of adenine nucleotides added to the 3' end of mRNA that aids in stability and export from the nucleus.

• a sequence of guanine nucleotides found at the 5' end of mRNA.

• a protein that binds to DNA to initiate transcription.

• an enzyme that removes introns from pre-mRNA.