Western Blotting Pretest

What is the primary purpose of Western blotting?

• To analyze RNA sequences in a sample.

• To visualize cell morphology under a microscope.

• To detect specific proteins in a sample.

• To measure DNA concentration in a sample.

Which of the following is used to separate proteins in Western blotting?

• Ion exchange chromatography

• SDS-PAGE

• Affinity chromatography

• Gel electrophoresis

What is the role of the primary antibody in Western blotting?

• The primary antibody enhances the signal of the secondary antibody.

• The primary antibody is used to block non-specific binding sites.

• The primary antibody is responsible for transferring proteins to the membrane.

• The primary antibody binds specifically to the target protein for detection.

Which step follows the transfer of proteins to a membrane?

• Folding and post-translational modifications of proteins.

• Protein degradation in the cytoplasm.

• Transport of proteins to the nucleus.

• Initiation of protein synthesis.

What is the purpose of blocking in Western blotting?

• To separate proteins based on size.

• To prevent non-specific binding of antibodies.

• To enhance the signal of the target protein.

• To increase the binding affinity of antibodies.

Which type of gel is commonly used for protein separation?

• Polyacrylamide gel

• Cellulose gel

• Agarose gel

• Silica gel

What is the significance of using a secondary antibody?

• The significance of using a secondary antibody is to amplify the signal and improve detection of the primary antibody-bound target.

• To reduce the cost of the experiment.

• To replace the primary antibody entirely.

• To provide a color change in the sample.

What detection method is often used in Western blotting?

• Mass spectrometry detection

• Antibody-based detection

• Enzyme-linked detection

• Fluorescent dye detection

What is the typical size range of proteins analyzed by Western blotting?

• 1 to 10 kDa

• 10 to 250 kDa

• 5 to 50 kDa

• 100 to 500 kDa

Which of the following is NOT a common application of Western blotting?

• Cell culture techniques

• Protein purification

• Enzyme activity assays

• DNA sequencing